Temporal Isolation — Definition & Examples

You are studying a population of jumping spiders. The spiders had been separated into two subpopulations by a storm. When you reintroduce the spiders to each other in the lab, they have difficulty interacting because one group is usually active at night and the other is usually active during the day. You give the daytime spiders a lot of caffeine so they will stay up late and discover that the daytime spiders prefer colorful mates and the nighttime spiders prefer mates that can jump long distances. What sequence of isolating mechanisms did the spiders experience?