Chromatography: Proteins

Chromatography means "color drawing" which originally referred to separating natural pigments on filter paper. Similarly in the context of proteins, it is a separation technique to isolate and identify proteins.

As explained in the video, the retention factor can be calculated by the distances that the analyte and solvent moved, thus identifying the protein's properties.

Although the video discusses an ascending paper method, there are several chromatography techniques used for purifying and detecting proteins:

Ion exchange chromatography (IEXC)

Chromatography based on hydrophobicity: hydrophobic interaction chromatography (HIC) and reversed phase chromatography (RPC)

Gel filtration or size-exclusion chromatography

Affinity chromatography

References:

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